Mobile phase cleaner for HPLC

Ghost Trap DS / Ghost Trap DS-HP (for UHPLC)

A new high-pressure model for the elimination of impurities from organic solvents has been added to the Ghost Trap DS* lineup.
The Ghost Trap DS* was co-developed with Daiichi Sankyo Co., Ltd. It has been designed to effectively adsorb impurities in the mobile phase in order to reduce the time required for method development and impurity analysis.

The 100 MPa pressure resistance of the Ghost Trap DS-HP allows it to be used with UHPLC systems. This permits the effective use of the Ghost Trap DS for method development by UHPLC and subsequent transfer to conventional analysis.

* DS: Abbreviation of Daiichi Sankyo (D) and Shimadzu (S)

Consistently traps impurities, even in organic solvents

A major feature of the Ghost Trap DS is the ability to remove impurities even in organic solvents. When the Ghost Trap DS is installed between the gradient mixer and autosampler in reversed phase gradient analysis, it can trap impurities from the piping and gradient mixer in addition to those from the mobile phase.
The example below shows that it can effectively trap impurities in mobile phase, when the Ghost Trap DS is installed just downstream of the gradient mixer as displayed in the figure on the right.

Column ODS column
Mobile phase A: 25 mmol/L Phosphate (Potassium) buffer solution pH 4.0 /Acetonitrile =9/1
B: Water/Acetonitrile = 1/9
Flow rate 0.65 mL/min
Oven 45 ºC
Detection UV 210 nm

Example of removing ghost peaks by Ghost Trap DS (The data is provided by Daiichi Sankyo Co., Ltd.)

Application to Quantitative Analysis of Impurities Using the Nexera UHPLC

Quantitation of a small peak covered by ghost peaks can be difficult. Effective for the quantitative analysis of impurities, the Ghost Trap DS/DS-HP effectively eliminates such ghost peaks to ensure highly accurate quantitation, even with very small target component peaks.

Analytical conditions

Mobile phase A 5 mmol/L phosphoric acid (sodium) buffer solution (pH 2.6)
Mobile phase B 5 mmol/L phosphoric acid (sodium) buffer solution (pH 2.6) / Acetonitrile = 1/3
Gradient 0.1 % (0 min) → 20 % (10 min) → 60 % (15 min) → 95 % (20 min)
Flow rate 0.5 mL/min
Detection UV245 nm
Injection volume 2 μL
Column Shim-pack XR-ODSⅢ 75 mmL. x 2.0 mmI.D., 1.6 μm

When the Ghost Trap DS/DS-HP is not used, quantitation of an impurity peak is
difficult due to the effects of ghost peaks. The Ghost Trap DS/DS-HP effectively
eliminates the ghost peaks to allow quantitation of the target component.


Two Ghost Trap DS models are now available: the cartridge type for superior cost performance and the 100 MPa high-pressure type for use with UHPLC systems. Select the appropriate one for your application.

Item P/N Description Internal volume *1 Dimensions Pressure
Ghost Trap DS 228-59921-91 Two cartridge 30 mmL. x 7.6 mmI.D. Approx. 700 μL 35 MPa

Two cartridges

and one holder

228-59921-93 Two cartridge 20 mmL. x 4.0 mmI.D. Approx. 150 μL

Two cartridges

and one holder

Ghost Trap DS-HP 228-59931-91 Packed type 30 mmL. x 2.1 mmI.D. Approx. 60 μL 100 MPa
  • *1 Note that a delay volume equivalent to the internal volume of the product occurs if the product is installed downstream of the gradient mixer or the confluence of two pumps.
  • * The product service life differs according to analysis conditions, such as the mobile phase used.
  • * In analysis using an ion-pairing reagent, the ion-pairing reagent may be retained in the product, influencing the retention time and peak shape.
  • * Before connecting the analytical column, be sure to thoroughly clean the flow path with mobile phase (close to the final concentration for gradient analysis).
  • * Note that some impurities may not be removed.
  • * When performing high-pressure analysis exceeding 35 MPa with a UHPLC system, connect the gradient mixer to the Ghost Trap DS-HP with pipe for UHPLC (e.g.228-53137-97).

For Research Use Only. Not for use in diagnostic procedures.

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