Analysis of herbal medicines by HPLC generally requires separation of the impurities and active constituents, so the time to required to complete an analysis becomes relatively long.
Sennosides, present in senna, display purgative action after decomposing in the intestine. For this reason, senna is used as a laxative.
Fig.1 shows an analysis example of sennosides A and B in senna.

Chromatogram of Senna
 

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